Auto-counter antibodies - through a Coombs direct test
General information:
Direct antiglobulin test (DAT or direct Coombs test, direct Coombs test) is used to prove antibodies fixed on the membrane of red blood cells - erythrocytes.
The test is used in the diagnosis of autoimmune hemolytic anemias, hemolytic disease of the newborn (CKD), reactions after blood transfusion (post-transfusion reaction).
Sensitized in vivo (in the body) erythrocytes with IgG and/or C (complement) class antibodies are agglutinated by polyvalent anti-human antiglobulin serum (PAGS).
The methods used are based on the principle of agglutination - column hemagglutination technology. The reading of the result is carried out on a four-level scale as the degree of agglutination.
The presence of agglutination indicates that the examined erythrocytes are sensitized with IgG and/or C (complement) class antibodies.
The presence of antibodies directed at the antigens of one's own erythrocytes is observed in:
- other autoimmune diseases, such as systemic lupus erythematosus, etc.
- malignant diseases - lymphoma, chronic lymphocytic leukemia, etc.
- infections - mycoplasma pneumonia, mononucleosis, etc.
- as a result of the administration of certain drugs, such as penicillin, etc.
Sample required:
Venous blood
Key words:
Auto-counter antibodies, coombs test